Fig. 5.

Glutaraldehyde (GA) cross-linking analysis of NtD and CtE. The purified proteins FL, NtD and CtE were cross-linking by glutaraldehyde and resolved on 10% SDS-PAGE. The shifts of protein band are indicated by the arrow. Lane M standard protein marker. a NtD and CtE were cross-linked by >0.002% GA. b NtD and NtD were cross-linked by >0.002% GA. c CtE and CtE could not be cross-linked by GA. d FL and FL were cross-linked by >0.002% GA. e The interaction between GST-fused CtE (GCtE) and NtD was further confirmed. The lower band indicates the interaction of NtD–NtD, and the higher band indicates the interaction of GCtE–NtD. f The results confirm the interaction between NtD and NtD but not NtD and GST