Fig. 3.

Over-expression of HMOX1, AKR1C2, AKR1C3 and AKR1B10 in C4-2B and PC-3M-luc2 prostate cancer cells after treatment with ethanol extract of neem leaves (EENL) for 24 and 48 h. Protein levels were measured with specific antibodies by Western blot analysis; GAPDH was the loading control. Vehicle-treated cells were used as control. The experiments were repeated thrice and the representative blot was shown