Fig. 1.

CD5 is downregulated during peripheral human CD8⁺ and CD4⁺ memory T-cell differentiation. (A) Density plots show expression of CD5 on human CD8⁺ memory T-cell subsets. Memory subsets were classified as central-memory (T_CM; CD45RA^–CCR7⁺), effector-memory (T_EM; CD45RA^–CCR7^–), and effector-memory CD45RA⁺ (T_EMRA; CD45RA⁺CCR7^–). Numbers indicate CD5 mean fluorescence intensity (MFI). Data are representative of 12 experiments. Bar graph shows CD5 MFI on human CD8⁺ memory T-cell subsets (n = 12). Statistical analysis was performed using Student's t test. ***p < 0.001, T_CM vs T_EM and ^###p < 0.001 T_CM vs T_EMRA. (B) Density plots show the expression of CD5 on human CD4⁺ memory T-cell subsets. Memory subsets were classified as described above. Numbers indicate CD5 MFI. Data are representative of eight experiments. Bar graph shows CD5 MFI on human CD4⁺ memory T-cell subsets (n = 8). Statistical analysis was performed using Student's t test. ***p < 0.001, T_CM vs T_EM and ^###p < 0.001 T_CM vs T_EMRA.