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Acute in vivo changes in local E2 levels in NCM are dependent on presynaptic VGCCs. Presynaptic inhibition of VGCCs via retrodialysis of ω-conotoxin disrupts the suppressive effect of potassium-evoked excitation on local E2 levels. N = 4/group; each time bin = 30 min. **p < 0.005 for within-subject comparison to pre-sampling period. Dashed line represents the average ELISA background reading for aCSF alone.
