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. 2011 Jul 25;194(2):257–275. doi: 10.1083/jcb.201012028

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© 2011 Fairn et al.

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Figure 5. — Distribution of PS in the endocytic pathway. A431 cells doubly transfected with GFP-Lact-C2 and RFP-Rab5 (a) or GFP-Lact-C2 and Lamp1-RFP (b), or transfected with GFP-Lact-C2, then incubated in the presence of tetramethylrhodamine (TMR) dextran for 16 h and chased for 1 h before visualization (c), as indicated. In a and b, arrows identify representative endosomal structures labeled by both markers. Inset shows enlarged views of the merged image. Bars, 5 µm.