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. 2011 Jul 25;194(2):307–322. doi: 10.1083/jcb.201007108

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© 2011 Brunner et al.

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Figure 8. — β1 integrin regulates in vitro mineralization in an Icap-1–dependent manner. β1^fl/fl (wild type), β1^WT (rescue), β1^D759A, β1^V787T, and β1^−/− cells were induced to differentiate into osteoblasts. Expression of alkaline phosphatase (ALP) was used to follow the early commitment of cells to the osteoblast lineage at day 0 (D0) and day 15 (D15). Mineralization was visualized by Alizarin red S staining (ARS) at day 20 (D20). Arrowheads indicate mineralized bone nodules. Bar, 1 mm.