Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jul 25;194(2):209–227. doi: 10.1083/jcb.201105010

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Ju et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 9. — Stimulation of the A_2AR rescued AMPK-mediated suppression of Bcl2 via a PKA–CREB-dependent pathway. (A) Mice were treated daily with CGS (2.5 mg/kg of body weight, i.p.) or vehicle for 6 wk from the age of 4 wk. Transcript levels of Bcl2 assessed by reverse transcription qPCR were normalized to those of GAPDH. a, P < 0.05 versus R6/2 mice. (B and D–F) Cells were transfected with the indicated constructs for ∼24–48 h and then treated with the indicated reagents for 24 h. Transcript levels of Bcl2 assessed by reverse transcription qPCR were normalized to those of GAPDH (B). a, P < 0.05 versus untreated STHdh^Q7 cells; b, P < 0.05 versus STHdh^Q109 cells treated with CGS; c, P < 0.05 with a specific comparison between cells transfected with CREB-WT and CREB-T133A; d, P < 0.05 versus untreated STHdh^Q109 cells; e, P < 0.05 versus AICAR-treated cells. (D–F) Cell survival was determined using an MTT assay (D and E) and flow cytometry–based analysis (F). (C and G–I) Cells were treated with the indicated reagents for 24 h. Levels of phosphorylated CREB (CREB-p^S133) were assessed by Western blot analyses and normalized to those of total CREB (C). Levels of AMPK-α-p^T172 and AMPK-α1 were assessed by Western blot analyses and normalized to those of actin (G–I). Molecular mass is indicated in kilodaltons. a, P < 0.05 versus untreated STHdh^Q109 cells; b, P < 0.05 versus untreated STHdh^Q7 cells. (A–I) Data are presented as the mean ± SEM of three independent experiments.