Figure 4.

Disruption of axonal accumulation of KIF5C by high-level expression of hMB11-mCherry. (A) hMB11-mCherry was initially dimly expressed around the cell body (0 min, red), but gradually covered whole neurons (arrow, 490 min, red). The asterisk at 0 min indicates cell debris. Truncated KIF5 (green), which initially accumulated at the tip of the axon (arrowheads, 460–610 min, green), went back into the cell body (730, 870 min, green). Images were enhanced to visualize neurites and, accordingly, cell body staining was saturated after 490 min. The intensity of original images of cell body staining of hMB11-RFP was 32 (460 min), 46 (510 min), and 86 (730 min) in arbitrary units. Bar, 20 µm. (B) Quantification of hMB11-mCherry expression level and localization of truncated KIF5 in stage 3 neurons (n = 55). hMB11 level in the cell body was quantified. Error bars indicate SEM. Bars, 10 µm.