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. 2011 Jul 6;31(27):10076–10087. doi: 10.1523/JNEUROSCI.0618-11.2011

Figure 2.

Figure 2.

Cytoplasmic accumulation of α-syn and neuronal loss in the cortex and hippocampus. A, Paraffin sections of the frontal cortex (frontal ctx), cingulate cortex (cing ctx), entorhinal cortex (entorh ctx), and hippocampal CA1 (hipp CA1) areas from 20-month-old tTA/A53Tα-syn mice were stained with anti-syn antibody, Syn 303 (n = 6). Boxed areas in the left panels indicate magnified areas in the right panels. Brain sections (coronal) from the control (tTA monogenic) or tTA/A53α-syn bigenic mice (n = 6) at the level of hippocampus were stained with hematoxylin and eosin (H and E) (B), or antibody against NeuN (brown) (C). Boxed areas in C indicate where the magnified images in the insets were taken. D, Diagrammatic summary of α-syn pathology distribution in tTA/A53Tα-syn mice. Blue dots represent synaptic/neuritic pathology, green dots represent cytoplasmic pathology, and red dots represent cell loss. Coronal sections of the one hemisphere are illustrated for simplicity (from left to right, each diagram is taken at the level of bregma 2.8 mm, 0.14 mm, −2.06 mm, −3.08 mm, and −5.68 mm based on the mouse brain atlas by Paxinos and Franklin, 2011). ctx, Cortex; DG, dentate gyrus; hipp CA1, hippocampal CA1. Scale bars: A, 25 μm; B, 500 μmm; C, 200 μm.