Table 3.
Uridine phosphorolysis assay results for SpUP and mutants
| SpUP | uridine cleavage activities (nmol/mg/h) |
|---|---|
| wild-type | 1,500,000 |
| K162A | < 20 |
| H169A | 112,000 |
| H169D | 9,000 |
| H169N | 42,000 |
| V220D | 550 |
| V220E | 3,400 |
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