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Steady State Kinetic Parameters for SpUP and EcUPa
| Enzyme | kcat (s−1) | Km (mM) | kcat/Km (mM−1 s−1) |
|---|---|---|---|
| SpUP | 15 ± 3 | 0.158 ± 0.025 | 95 ± 24 |
| EcUP | 23 ± 1 | 0.036 ± 0.004 | 639 ± 76 |
| SpUP-H169A | 1.7 ± 0.2 | 0.171 ± 0.005 | 9.9 ± 1 |
| SpUP-K162Ab | - | - | N/A |
The concentration of uridine was varied and the production of uracil was monitored.
No activity was observed at the level of detection of the assay.
