Table 2.
Immunohistochemistry and microdialysis experiments
Experimental days | BDNF immunohistochemistry |
Microdialysis |
||
---|---|---|---|---|
Stressed | Controls | Stressed | Controls | |
Episodic social defeat stress | ||||
5 d before first social defeat | Guide cannula implanted in NAC | |||
Days 1, 4, 7, and 10 | Defeated in resident encounters | Handled | Defeated in resident encounters (n = 7) | Handled (n = 6) |
Day 21 | Injected with cocaine or saline 60 min before perfusion | Injected with saline and then cocaine during microdialysis | ||
Continuous subordination stress | ||||
Days 1–36 | Housed in home cage of aggressive male | Handled | Housed in home cage of aggressive male (n = 6) | Handled (n = 6) |
Day 37 | Guide cannula implanted in NAC | |||
Day 47 | Injected with cocaine or saline 60 min before perfusion | Injected with saline and then cocaine during microdialysis |