FIGURE 3.
Designed PUF domains that recognize targets with multiple cytosines. A, diagram showing the mutations in PUM repeats 2 and 6 to recognize C3C7 RNA (left). Relative protein-RNA binding is shown as in Fig. 2 (right). B, stepwise generation of a PUF mutant (PUF-D) that can bind to (CUG)n repeat RNA. Diagrams show the mutations in PUM repeats 1, 3, 4, 5, and 6 (center, PUF-D) or in PUM repeats 1, 2, 3, 5, 7, and 8 (right, PUF-E) to recognize (CUG)n repeat RNA. C, relative protein-RNA binding to WT or (CUG)5 RNA is shown as in Fig. 2. Error bars indicate S.D.