FIGURE 6.

Natural PUF proteins with putative cytosine-recognition code. A, alignment and phylogenetic tree of the putative C-recognition PUM repeat in Nop9p homologs from yeast, plants, filamentous fungi, and protists. The query sequences were selected to maximize the divergence of the species but are otherwise arbitrary. The Giardia protein EES98274 was the chosen as the outgroup in the phylogenetic tree. B, alignment of the putative C-recognition PUM repeat in Nop9p homologs from the HomoloGene database. The homologous Volvox carteri protein XP_002952190 was included in the alignment as the outgroup in the phylogenetic tree. The conserved positions for cytosine recognition were highlighted.