TABLE 1.

Nucleotide sequences recovered from the Y3H screen

In total, 20 independent clones were sequenced, and the resulting codons are listed with the encoded amino acid residue in parentheses. The residue at position 1043 (position 2 in the 5-residue RNA-interaction motif) is tyrosine for all clones. Clone 18 did not have an unambiguous sequence for the first codon (indicating either an A or C in the second position) and thus was disregarded in our analyses.

	AA position 1043	AA position 1047
Wild type	AAT(Asn)	CAA(Gln)
1	AGT(Ser)	AGA(Arg)
2	AGT(Ser)	AGA(Arg)
3	AGT(Ser)	AGG(Arg)
5	AGT(Ser)	AGA(Arg)
6	AGT(Ser)	CGC(Arg)
7	AGT(Ser)	AGG(Arg)
8	TCC(Ser)	CGA(Arg)
9	AGT(Ser)	CGG(Arg)
10	AGT(Ser)	CGG(Arg)
11	AGT(Ser)	AGA(Arg)
12	AGT(Ser)	AGG(Arg)
13	TCT(Ser)	AGG(Arg)
14	TCA(Ser)	CGT(Arg)
15	AAT(Asn)	TAG (stop)
16	AGT(Ser)	AGG(Arg)
17	AGT(Ser)	AGA(Arg)
18	A(A C)T(Asn Thr)	CGG(Arg)
19	AGT(Ser)	AGA(Arg)
20	AGT(Ser)	AGG(Arg)