Sequential changes in the serum lipid status and hepatic enzyme. (a) The levels of triglyceride between three groups had a significant interaction (P = 0.001). In high-dose alcohol (HDA) group, the levels of triglyceride were significantly increased at 1 (†P = 0.005) and 2 weeks (‡P = 0.001) in comparison with those at week 0. At weeks 1, 2 and 3, the triglyceride levels in the low-dose alcohol (LDA) group were significantly higher than those in the physiologic saline (PS) group (* to ***P < 0.05). At weeks 1, 2, 3 and 4, the triglyceride levels in the HDA group were significantly higher than those in the PS group (*P = 0.001, **P = 0.001, ***P < 0.01 and ****P < 0.05 respectively). (b) The free fatty acid levels between three groups had a significant interaction (P = 0.001). In HDA group, the levels of free fatty acids were significantly increased at 1 (†P = 0.002), 2 (‡P = 0.001) and 3 weeks (‡P = 0.001) in comparison with those at week 0. At weeks 1 and 2, the levels of free fatty acids in the LDA group were significantly higher than those in the PS group (*P = 0.01 and **P < 0.05 respectively). At weeks 1, 2, 3 and 4, the levels of free fatty acids in the HDA group were significantly higher than those in the PS group (*P < 0.005, **P = 0.001, ***P < 0.05 and ****P < 0.05 respectively). (c) The low-density lipoprotein (LDL)/high-density lipoprotein (HDL) cholesterol ratio between three groups had no significant interaction (P = 0.406). In HDA group, the LDL/HDL cholesterol ratio was significantly increased at 2 weeks (†P < 0.05) in comparison with week 0. At weeks 3 and 4, levels of LDL/HDL cholesterol ratios in the HDA group were significantly higher than those in the PS group (*P < 0.05 and **P < 0.05 respectively). (d) The levels of aspartate aminotransferase (AST) between three groups had no significant interaction (P = 0.091). In HDA group, the levels of AST were significantly increased at 1 week (†P < 0.05) in comparison with those at week 0. At week 1, the AST level in the HDA group was significantly higher than that in the PS group (*P < 0.05).