Table 2.
TP receptor ligand association kinetics
| Bmax | Kd | |
|---|---|---|
| TP | ||
| Normoxic | 412 ± 7.2 | 8.8 ± 0.21 |
| Hypoxic | 315 ± 7.6 | 6.55 ± 0.05** |
| TP + Iloprost | ||
| Normoxic | 342 + 6.0 | 11.43 + 0.28 |
| Hypoxic | 361 + 9.4 | 10.29 + 0.28 |
Mean kinetics for TP receptor ligand association in hypoxic and normoxic myocyte cell membrane fractions, using the radiolabelled TP receptor antagonist 3H-SQ29548. Dissociation constant (Kd) was lower in hypoxic than in normoxic myocytes (**P < 0.01; data represent three separate experiments). Pre-incubation of membrane fractions with 1 µM iloprost increased Kd of both hypoxic and normoxic cells, eliminating the difference between the groups (P = ns; data from three separate experiments).