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. 2011 Jul;163(6):1263–1275. doi: 10.1111/j.1476-5381.2011.01310.x

Figure 2.

Figure 2

In vitro metabolism of N-(4-hydroxyphenyl)retinamide (4-HPR) and N-(4-methoxyphenyl)retinamide (4-MPR) in pooled human (HLM) and mouse liver microsomes (MLM). (A) Rate of 4-oxo-N-(4-hydroxyphenyl)retinamide (4-oxo-4-HPR) formation from 4-HPR in HLM and (B) in MLM. (C) Rate of demethylation of 4-MPR to the parent drug in HLM. (D) Rate of 4-oxo-4-HPR formation from 4-MPR in HLM. (A–D) Substrates at indicated concentrations were incubated in HLM or MLM for 60 min. Points in each graph are the mean and error bars are standard deviation from experiments performed in triplicate. Amounts measured by HPLC were normalized to the amount of HLM or MLM protein. In each graph, the Km (µM) and Vmax (nmol·h−1 mg protein−1) are the mean ± SD.