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. 2011 Jul 5;2:376. doi: 10.1038/ncomms1377

Figure 7. Analysis of GAD65−/− interneurons.

Figure 7

(a) Summary of Ihold recorded in WT (shaded columns) and GAD65−/− (open columns) interneurons voltage-clamped at −60 mV loaded with CsCl (GAD65−/− n=8; WT n=13) and with KCl (GAD65−/− n=5; WT n=7) in the presence of 100 μM picrotoxin. (b) Cell-attached recordings from WT and GAD65−/− interneurons (without picrotoxin). (c) Summary plot of firing frequency in WT and GAD65−/− interneurons (n=7) obtained under the same conditions as for b. (d) Left panel: Action potentials recorded in response to depolarizing current steps (90 pA; 500 ms) in whole-cell current-clamp mode from WT and GAD65−/− interneurons in the presence of 100 μM picrotoxin. Right panel: Summary plot of action potential frequency recorded during depolarizing steps varying from 30 to 180 pA (input–output characteristics) in GAD65−/− (n=5) and WT (n=4) interneurons. (e) Cell-attached recordings from GAD65−/− interneuron in control (ctrl) and in 1 and 5 μM GABA (without picrotoxin). (f) Summary plot of the change in firing frequency induced by GABA in GAD65−/− interneurons obtained under the same conditions as for e. Error bars, s.e.m., **P<0.005, *P<0.05, NS P>0.05, unpaired (a and c) and paired (f) t-test.