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Embryonic pigment cells in a blastula-derived cell culture of the sea urchin S. intermedius. (a) Multilayer cell sheets (2-3 days of cultivation in seawater supplemented with 2% fetal bovine serum); (b) spread pigment cells cultivated in the coelomic fluid of control sea urchins for 3 days; (c) rounded pigment cells cultivated in the coelomic fluid of injured sea urchins for 3 days. Bar, 10 μm.
