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. Author manuscript; available in PMC: 2011 Jul 27.
Published in final edited form as: Methods Enzymol. 2009;468:239–258. doi: 10.1016/S0076-6879(09)68012-5

Figure 3.

Figure 3

X-ray footprinting of ribosomes in intact E. coli. Primer extension of purified 70S ribosomes (A) or rRNA isolated from irradiated cells (B). Exposure times are given at the top of the gel. Radiolabeled primer anneals after nt 560 of the 16S rRNA. Product bands were assigned by comparison with dideoxy sequencing lanes. Redrawn from (Adilakshmi et al. 2006).