Fig. 4.

Prolonged exposure to recombinant Tat(1-86) (100 nM) produced significant cell death and/or injury in the granule cell layer of the dentate gyrus after 24 and 72 h of exposure, but not after 120 h of exposure. Co-exposure to Trolox (500 μM) significantly reduced PI uptake induced by Tat after 24 and 72 h exposure in organotypic hippocampal cultures. Exposure to Trolox alone resulted in slight, but significantly, less PI uptake than control in the DG at 120 h, but did not affect PI uptake compared to control in the CA3 or CA1 regions. The dashed line represents control values. *P<0.001 vs. control; **P<0.001 vs. Tat within each respective timepoint