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. 2011 Jun 30;67(Pt 7):768–774. doi: 10.1107/S1744309111017209

Figure 3.

Figure 3

SDS–PAGE analysis of the purification of RPTPγ(825–1128)/(V948I, S970T) from E. coli. Samples were electrophoresed on a 4–20% Tris–glycine gradient gel and stained with Coomassie Blue. Lane 1, molecular-mass markers (kDa); lane 2, crude lysate (10 µg); lane 3, purified His-Tb-RPTPγ(825–1128)/(V948I, S970T) following nickel-affinity chromatography (5 µg); lane 4, purified His-Tb-RPTPγ(825–1128)/(V948I, S970T) following SP-Sepharose chromatography (5 µg); lanes 5, 6 and 7, purified RPTPγ(825–1128)/(V948I, S970T) following thrombin cleavage, Benzamidine Sepharose and size-exclusion chromatography (2, 4 and 8 µg, respectively).