Table 1.

Plasmodium vivax gametocyte stage- and sex-specific gene primers^*

Primer or probe	Sequence
pvs230 F	5′-TCAGTACAACCACCTGTTCAGCCA-3′
pvs230 R	5′-ACAAGTCCACGAGCTTCTTCACCT-3′
pvs230 probe	5′-6-FAM-AAATCGTCCCGCACAACTGCTTTGCCT-BHQ-3′
pvs28 F	5′-AACTGTGGAGACTACGCTGTGTGT-3′
pvs28 R	5′-ACATGGTGCTGTTCACATTAGCGG-3′
pvs28 probe	5′-6-FAM-AACGGCGTTCTGTGTGGAAAGGGAAA-BHQ-3′
alpha tubulin II F	5′-CTAGGGAAGGTACTCACAGATACG-3′
alpha tubulin II R	5′-CCCGACTGTCTATAAACTCTGC-3′
alpha tubulin II probe	5′-6-FAM-TGCTGGCCATCACCTCAAGTATCA-BHQ-3′
Pfg377 F	5′-CTGTACAGGTCTACAAGGCTTC-3′
Pfg377 R	5′-GAGTTGTATGGTTCCACCACAG-3′
Pfg377 probe	5′-6-FAM-ATAACTCCTACGATGCGGCGAAGAAG-BHQ-3′
Pfs25 F	5′-TCTTGTACATTGGGAACTTTGCCT-3′
Pfs25 R	5′-TGCGAAAGTTACCGTGGATACTG-3′

^*Primers and probes were used to determine expression of the following P. vivax sexual stage-specific genes: pvs230 (PVX_003905), pvs28 (PVX_111180), microgametocyte-specific gene alpha tubulin II (ATii, PVX_098630), and macrogametocyte-specific gene pvg377 (PVX_101400). BHQ = Black Hole Quencher. All primers and probes were obtained from Integrated DNA Technologies (San Diego, CA).