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Test of the sensitivity of the species-specific polymerase chain reaction (PCR) assay (all PCR conducted with 1 μL of DNA). Lanes 1 and 2 contain DNA from Aedes aegypti alone and lanes 3 and 4 from albopictus alone, lanes 5 and 6 contain DNA from Aedes aegypti and albopictus in equal amounts, lanes 7–10 contain DNA from Ae. albopictus and aegypti in 1/10 and 1/100 (2 lanes each) and lanes 11–14 contain DNA from Ae. aegypti and albopictus in the same ratios.
