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. 2011 Jul 27;6(7):e21812. doi: 10.1371/journal.pone.0021812

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Bastianelli et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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In vitro synchronized culture of P. falciparum composed of 0.5% of segmented schizonts was incubated for 12 hr in serum-containing medium and schizonts transition to newly formed trophozoites was analyzed by flow cytometry. Gates were converted to two-dimensional plots illustrating the expected merozoites egress defect in presence of 10 M E64 [51], but also in presence of 200 M of PcFK1. 91.6% and 56.2% of inhibition of newly formed trophozoites and equivalent segmented schizont accumulation were observed with 10 M E64 and 200 M PcFK1 respectively, when compared to the mock control. Mock condition corresponds to a classical P. falciparum culture in presence of 2% DMSO, the vehicle of PcFK1.

Inline graphic — In vitro synchronized culture of P. falciparum composed of 0.5% of segmented schizonts was incubated for 12 hr in serum-containing medium and schizonts transition to newly formed trophozoites was analyzed by flow cytometry. Gates were converted to two-dimensional plots illustrating the expected merozoites egress defect in presence of 10 M E64 [51], but also in presence of 200 M of PcFK1. 91.6% and 56.2% of inhibition of newly formed trophozoites and equivalent segmented schizont accumulation were observed with 10 M E64 and 200 M PcFK1 respectively, when compared to the mock control. Mock condition corresponds to a classical P. falciparum culture in presence of 2% DMSO, the vehicle of PcFK1.