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. 2011 Jul 27;6(7):e22359. doi: 10.1371/journal.pone.0022359

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Pereira et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fluorimetric analysis using Nile Red reveals that epimastigotes cultivated in 50% FCS store more neutral lipids than those grown in 10 and 1% FCS. Fluorescence intensity was expressed in arbitrary units. The results are from two independent experiments in triplicate. The data was analyzed with One-way ANOVA test (P<0.05).