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. 2011 Jul 27;6(7):e22582. doi: 10.1371/journal.pone.0022582

Table 1. Crystallization times, crystal sizes and nucleation densities for lysozyme in the presence of layered silicates.

Layered silicate Time Size Density
Sample name Idealized structural formula (day) (mm) (n.c./mm2)
Control - 2 0.44±0.11 0.49±0.31
Pyrophyllite Al2Si4O10(OH)2 6 0.62±0.15 0.30±0.16
Hectorite Na0.33Mg2.67Li0.33Si4O10(OH)2 5 0.78±0.09 0.15±0.04
Talc Mg3Si4O10(OH)2 4 0.82±0.09 0.15±0.02
Muscovite KAl3Si3O10(OH)2 4 0.74±0.14 0.17±0.07
Saponite Na0.33Mg3Al0.33Si3.67O10(OH)2 4 0.52±0.14 0.34±0.19
Montmorillonite Na0.33Mg0.33Al1.67Si4O10(OH)2 3 0.72±0.14 0.21±0.14
Sericite KAl3Si3O10(OH)2 3 0.71±0.13 0.21±0.07
Fluorophlogopite KMg3AlSi3O10F2 1.5 0.30±0.12 1.71±1.11
Fluorohectorite Na0.33Mg2.67Li0.33Si4O10F2 1.5 0.29±0.12 2.14±1.33
K-tetrasilicic fluoromica KMg2.5Si4O10F2 1.5 0.28±0.08 2.72±1.52
Na-tetrasilicic fluoromica NaMg2.5Si4O10F2 1.3 0.08±0.03 25.0±12.4

Crystallization conditions: starting lysozyme concentration 20 mg/mL, starting silicate content 0.2%, precipitant agent sodium chloride 1.0 M in sodium acetate 0.2 M, pH 4.7. Crystallization time was defined as the time that elapses before the appearance of the first crystal. Crystal size refers to the average length of the longitudinal axis of the crystal calculated on a set of several hundreds of crystals. Nucleation density is expressed as the number of crystals per mm2. Reported values are mean ± SD, n>40.