Table 1. Crystallization times, crystal sizes and nucleation densities for lysozyme in the presence of layered silicates.
Layered silicate | Time | Size | Density | |
Sample name | Idealized structural formula | (day) | (mm) | (n.c./mm2) |
Control | - | 2 | 0.44±0.11 | 0.49±0.31 |
Pyrophyllite | Al2Si4O10(OH)2 | 6 | 0.62±0.15 | 0.30±0.16 |
Hectorite | Na0.33Mg2.67Li0.33Si4O10(OH)2 | 5 | 0.78±0.09 | 0.15±0.04 |
Talc | Mg3Si4O10(OH)2 | 4 | 0.82±0.09 | 0.15±0.02 |
Muscovite | KAl3Si3O10(OH)2 | 4 | 0.74±0.14 | 0.17±0.07 |
Saponite | Na0.33Mg3Al0.33Si3.67O10(OH)2 | 4 | 0.52±0.14 | 0.34±0.19 |
Montmorillonite | Na0.33Mg0.33Al1.67Si4O10(OH)2 | 3 | 0.72±0.14 | 0.21±0.14 |
Sericite | KAl3Si3O10(OH)2 | 3 | 0.71±0.13 | 0.21±0.07 |
Fluorophlogopite | KMg3AlSi3O10F2 | 1.5 | 0.30±0.12 | 1.71±1.11 |
Fluorohectorite | Na0.33Mg2.67Li0.33Si4O10F2 | 1.5 | 0.29±0.12 | 2.14±1.33 |
K-tetrasilicic fluoromica | KMg2.5Si4O10F2 | 1.5 | 0.28±0.08 | 2.72±1.52 |
Na-tetrasilicic fluoromica | NaMg2.5Si4O10F2 | 1.3 | 0.08±0.03 | 25.0±12.4 |
Crystallization conditions: starting lysozyme concentration 20 mg/mL, starting silicate content 0.2%, precipitant agent sodium chloride 1.0 M in sodium acetate 0.2 M, pH 4.7. Crystallization time was defined as the time that elapses before the appearance of the first crystal. Crystal size refers to the average length of the longitudinal axis of the crystal calculated on a set of several hundreds of crystals. Nucleation density is expressed as the number of crystals per mm2. Reported values are mean ± SD, n>40.