Figure 1. Prophylactic therapy against lethal challenge by PR8 in mice.

Prophylactic therapy was performed by i.n. administration of Ad5 particles shortly before PR8 challenge. AdE/in/-2 and AdE*/in/-2, i.n. administration of AdE on day -2; AdE/in/+1, i.n. administration of AdE 1 day post-PR8 challenge; AdE/im/-2, i.m. injection of AdE on day -2; AdNC/in/-2 and AdNC*/in/-2, i.n. administration of AdNC.H1.1 on day -2; AdNC/im/-2, i.m. injection of AdNC.H1.1 on day -2; untreated control, Balb/c mice without treatment prior to PR8 challenge; all groups were inoculated with AdE or AdNC.H1.1 at a dose of 1.7×10⁸ ifu except AdE*/in/-2 and AdNC*/in/-2 groups that received a dose of 1.7×10⁶ ifu; all groups were challenged by i.n. instillation of 4xLD₅₀ of PR8 on day 0; body weights were recorded daily for 18 days post-challenge with 30% body weight loss taken as the disease endpoint; numbers in parentheses represent the number of animals in each group.