Figure 2. Simultaneous imaging of brain tumor angiogenesis and invasion with μMRI.

(a) FA map of a patient-derived, invasive primary glioma model. (b) Zoomed view of the hatched region in (a) showing two ROIs in the corpus callosum for which the FA was analyzed. (c) Histograms of the FA from the ROIs in (b), wherein one can see that the FAs from ROI-1 are shifted toward lower values than those from ROI-2. (d) Histology (H&E) from the same region as in (b) in which one can see the white matter tract (WM) being infiltrated by a tuft of tumor cells (I). The tumor margin (T) is also visible in (d). (e) Visualization of the DTI tensors as 3D ellipsoid glyphs for one μMRI slice, wherein each ellipsoid is scaled according the values of the three principal eigen-vectors and color coded according to the FA. The invasive primary tumor (hatched outline) is identifiable by its lower FA in contrast to the contralateral brain. (f) Visualization of the 3D vasculature for the whole brain. Tumor vasculature (hatched outline) is dense and chaotic relative to that of the contralateral brain. (g) The image in (e) overlaid with that in (f) allows us to simultaneously assess the interaction between brain tumor angiogenesis and the effects of tumor invasion on the integrity of white matter tracts. The tumor ROI is highlighted by a hatched outline.