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. 2011 Jul 27;6(7):e22643. doi: 10.1371/journal.pone.0022643

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Pathak et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) In vivo macrovascular CBV (ΔR₂*) map. (b) Co-registered ex vivo fractional blood volume (FV) map obtained from μMRI. The tumor ROI is highlighted by hatched lines in each panel and FV ranges from 0 to 1. (c) Histograms showing the relative distribution of the ΔR₂* between tumor and contralateral ROIs. (d) Histograms showing the relative distribution of the FV between tumor and contralateral ROIs. Tumor blood volume is elevated relative to the contralateral brain across these “multi-scale” data. (e) 2D histograms of the macrovascular CBV measured in vivo versus the fractional blood volume assessed ex vivo. These data further demonstrate the utility of multi-scale imaging of brain tumor vascularization.