Representative DHE staining and quantified data showing no difference in baseline ROS levels in the PVN (A, B) or RVLM (C, D) between KO and NT mice (P>0.05, n = 8). AngII infusion significantly increased ROS production in these regions in both genotypes (A–D; *P<0.05 vs. baseline, n = 8), with higher levels in KO (A–D; †P<0.05 vs. NT+AngII). Baseline AngII levels in both plasma (E) and brain (F) were not different between mice (P>0.05, n = 8). While AngII infusion significantly increased plasma (E) AngII levels in both genotypes, only KO mice show elevated brain AngII levels (F) (*P<0.05 vs. baseline, n = 8). KO mice exhibited higher plasma and central AngII levels following AngII infusion (†P<0.05, vs. NT+AngII).