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. 2011 Jul 27;6(7):e22809. doi: 10.1371/journal.pone.0022809

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Guy11, ΔMoglk1 and ΔMohxk1 mutants were grown on MM containing 1% glucose and 100 mM ammonium. Photographs (A) were taken from one transformant of each mutant at indicated days after incubation at 30°C. Colony diameter (B) and medium pH (C) were measured at the same days. The experiment was performed with three strains for the ΔMoglk1 and ΔMohxk1 mutants, respectively; and three independent replicates provided the same results. Different letter represent difference (P<0.05) among Guy11 and ΔMoglk1, ΔMohxk1.