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. 2011 Jul 27;6(7):e22735. doi: 10.1371/journal.pone.0022735

Figure 3. Oligodendrocyte depletion with DT results in axonal damage but does not affect cortical neuronal density.

Figure 3

(A) A decrease of neurofilament signal density (NF200), and an increase in axonal damage (evidenced by APP accumulation) was observed in MOGi-Cre/iDTR mice treated with DT (left panel), compared to DT-treated MOGi-Cre animals as control (middle panel) and MOGi-Cre/iDTR animals treated with PBS (right panel). Quantifications are shown as mean and SEM (n = 3–10). One way ANOVA was performed, followed by pairwise Tukey test. Significance is represented with asterisks (**p<0.01, ***p<0.001). Scale bar, 100 µm. (B) Coronal sections were stained for neuronal nuclei with NeuN antibodies, scanned with Mirax Midi System and automatically counted using Definiens Developer XD software in the indicated region of interest (ROI). One way ANOVA was performed, no statistically significant difference in neuronal density was observed between the groups. Also, no significant difference was found in cortical volume between the groups (p>0.05). An average of 10104 NeuN+ were counted per animal. Quantifications represent mean and SEM (n = 8 MOGi-Cre/iDTR mice treated with DT, 5 DT-treated MOGi-Cre animals as control (middle panel) and 3 MOGi-Cre/iDTR animals treated with PBS, ns: not significant).