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. 2011 Jul 27;6(7):e22957. doi: 10.1371/journal.pone.0022957

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Fu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Electrophoretic analysis of PCR products amplified from flounder genomic DNA by the primer pairs designed on the basis of miRNA-5p and miRNA-3p based on similarity to miRNA stem-loop sequences in metazoon. For each miRNA, the left lane is the test sample and the right lane is the negative control. (B) Sequence alignment analysis of two miRNA precursors that have both conserved star and mature sequences. The alignment of mir-22a and mir-124 in different species shows high conservation of their mature miRNA and miRNA*. The green nt represent miRNA* sequence and the red represent mature miRNA sequence. The asterisks indicate the conserved sites among these species. dre, Danio rerio; fru, Takifugu rubripes; gga, Gallus gallus; xtr, Xenopus tropicalis; has, Homo sapiens; mmu, Mus musculus; pol, Paralichthys olivaceus; tni, Tetraodon nigroviridis; bp, base pairs; nt, nucleotides.