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. 2011 Jul 27;6(7):e22957. doi: 10.1371/journal.pone.0022957

Figure 3. Validation of miRNAs by Quantitative real-time PCR and Northern hybridization.

Figure 3

(A) Lift side: Expression levels of four miRNAs (mir-10d, -7, -10c, and -22a) at the flounder metamorphic stage. Different letters indicate significant differences between the corresponding metamorphic stages, p<0.05. Right side: Sequencing reads of four miRNAs by Solexa sequencing. (B) Relative expression levels of four miRNAs (mir-22a, -10d, -10c, and -7) at four different developmental stages (13, 21, 29, and 42 dph) of Japanese flounder, respectively. Different letters indicate significant differences between the corresponding metamorphic stages in four various miRNAs, p<0.05. (C) Expression analysis of eight miRNAs by Northern hybridization at the flounder metamorphic stage. dph, day post hatching; bp, base pairs.