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. 2011 Jul 27;6(7):e22957. doi: 10.1371/journal.pone.0022957

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Fu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Differential expression of miRNAs at two metamorphic stages (17 and 29 dph) by hierarchical clustering. Upside: Differential expression of 26 miRNAs at the two stages (p<0.01 and signal >500). Downside: Differential expression of 40 miRNAs at the two stages (p<0.01 and signal <500). Red indicates that a gene is highly expressed at that stage, whereas green indicates the opposite. The absolute signal intensity ranges from −1.5 to +1.5, with corresponding color changes from blue to green, yellow and red. The signal of expression was detected by microarray with three probe repeats. (B) Relative detectable signals of 26 differentially expressed miRNAs (p<0.01 and signal >500) at two metamorphic stages (17 and 29 dph) by microarray. The signals are average (of three replicates) absolute intensity. (C) Relative expression levels of six miRNAs (mir-1, -133a, -206b, -10d, -10b and -22a) at two metamorphic stages (17 and 29 dph) by Quantitative real-time PCR. ^* p<0.05 means a statistically significant difference between this miRNA levels at 17 and 29 dph.