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. 2011 Jul 27;6(7):e22811. doi: 10.1371/journal.pone.0022811

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Osuala et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The line of separation originates from the apex and extends toward the base of the heart and through the aorta (bold line). Additional partitioning isolates the IVS from the RV and LV free walls (panel b and d). Each partition was then analyzed to determine the total pixels containing the blue XGAL+ stain. The left heart shows heavy XGAL+ staining when compared to the right side of the heart. Pnmt^+/+, ROSA26^βgal/βgal control heart shows no XGAL+ staining (panel c and d).