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. 2011 Jul 27;6(7):e22811. doi: 10.1371/journal.pone.0022811

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Osuala et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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XGAL+ staining in the left heart reveals the anatomical location of cells which have historically expressed the Pnmt gene. Pnmt expression can be seen in the LA and the apical border of the LV ( panel a). Pnmt expression is seen distributed throughout the LA and can be seen within the cardiomyocytes under higher magnification (b, c). Pnmt expression is also observed in the free wall of the LV and appears to be within the cardiomyocytes, based on anatomical and morphological characteristics (d–f).