Skip to main content
. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Mol Carcinog. 2011 Apr 22;51(3):257–267. doi: 10.1002/mc.20780

Figure 5.

Figure 5

SHR-5 and salidorside induce autophagy in bladder cancer UMUC-3 cells. A: UMUC-3 Cells were treated with vehicle control, 50 μM Chloroquine, 200 μg/ml SHR-5, or 50 μg/ml salidroside for 8 hours. After treatments, cells are fixed and examined by an electronic microscopy for the presences of autophagosomes. B: UMUC-3 Cells stably expressing pEGFP-LC3 were treated with vehicle control, 50 μM Chloroquine, 100 and 200 μg/ml SHR-5, or 25 and 50 μg/ml salidroside for 8 hours. After treatments, cells were examined by a fluorescence microscopy. C: Quantitative analysis of percentage of UMUC-3 cells with LC3-puncta. Bar: mean ±SE. D: Western blotting analysis of p62.