Oligomycin and FCCP titrations for organotypic rat hippocampal slices. Absolute (A) and baseline-normalized (B) O2 consumption rates (OCR) of 14 DIV organotypic rat hippocampal slices exposed to successive additions of respiratory modulators (arrows) are shown. Slices received three additions of oligomycin (1 μg/ml each, open circles), FCCP (1 μM each, filled gray circles), or control (75 μl of aCSF each, filled black circles) followed by a final addition of antimycin A (10 μM, open and filled gray circles) or control (75 μl of aCSF, filled black circles). Rates in B are normalized to the third baseline measurement point. Data are mean ± SD, n = 4 slices per trace.