Oligomycin and FCCP titrations for acute rat hippocampal slices. Baseline-normalized O2 consumption rates (OCR) of acute rat hippocampal slices exposed to successive additions of respiratory modulators (arrows) are shown without (A) and with (B) exclusion criteria (see text). Rates are normalized to the third baseline measurement point. Slices received three additions of oligomycin (1 μg/ml each, open circles), FCCP (1 μM each, open squares or 5 μM each, filled squares), or control (75 μl aCSF each, filled circles) followed by a final addition of antimycin A (10 μM, open circles and open and filled squares) or control (75 μl aCSF, filled circles). Data in A are mean ± SD, n = 3, 3, 2, and 2 slices per trace for filled circles, open circles, filled squares, and open squares, respectively. In B, one slice per trace was excluded with the exception of filled squares.