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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Nat Genet. 2011 Jul 3;43(8):776–784. doi: 10.1038/ng.891

Figure 1. Tctn1 is required for ciliogenesis in a tissue-dependent manner.

Figure 1

(a) Wild type and Tctn1−/− E8.5 nodes stained for Arl13b (green) and DNA (DAPI, blue). Scale bar 10μm. (b) SEM of wild type and Tctn1−/− E8.0 nodes. Scale bars are 50μm, 5μm and 0.5μm in left, middle and right panels, respectively. (c) TEM of wild type and Tctn1−/− E8.5 nodes. Arrows indicate basal bodies. Scale bar 1μm. (d) Wild type and Tctn1−/− E10.5 ventral neural tube stained for Arl13b (green), γ-tubulin (red), and DNA (DAPI, blue). Scale bar 10μm. (e) SEM of wild type and Tctn1−/− E9.5 neural tubes. Arrowheads indicate cilia. Scale bars 1μm. (f) TEM of wild type and Tctn1−/− E9.5 neural tubes. Scale bar 1μm. (g) SEM of notochord cilia from wild type and Tctn1−/− E8.0 embryos. Scale bar 1μm. (h) E10.5 perineural mesenchyme sections were stained for AcTub (blue), Arl13b (red), γ-tubulin (green), and DNA (grey). Arl13b localization to Tctn1−/− cilia is reduced. Arrowheads indicate cilia. Scale bar 5μm. (i) E11.5 hindlimb bud mesenchyme sections were stained as in (h). Arl13b is also reduced in Tctn1−/− cilia. Arrowheads indicate cilia. Scale bar 5μm. (j) Alcian blue staining of wild type and Tctn1−/− E14.5 hindlimb cartilage. (k) In situ hybridization of E10.5 or E11.5 hindlimb buds for expression of the indicated genes. (l) In situ hybridization of E10.5 hindlimb buds for Fgf4 expression reveals that Tctn1 is epistatic to Shh. (m) Immunoblot of E9.5 wild type, Tctn1+/− and Tctn1−/− embryo extracts with Gli3 antibodies.