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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Nat Genet. 2011 Jul 3;43(8):776–784. doi: 10.1038/ng.891

Figure 5. Tctn1 interactors Cc2d2a and Tmem67 promote ciliogenesis.

Figure 5

(a) Control and Cc2d2a−/− E10.5 mouse embryo littermates. (b) Control and Cc2d2a−/− E9.5 ventral neural tube stained for Arl13b (red) and DNA (DAPI, blue). (c) Control and Cc2d2a−/− E9.5 perineural mesenchyme stained for Arl13b (red) and DNA (DAPI, blue). (d) Cc2d2a−/− primary MEFs generate cilia, marked by AcTub (green), with associated basal bodies, marked by γ-tubulin (red). (e) Hematoxylin and eosin staining of E18.5 kidney sections from control and Tmem67−/− embryos. Kidney cysts are visible in the mutant (arrows). (f) Control and Tmem67−/− E18.5 embryonic kidney tubules stained for AcTub (green), Arl13b (red) and DNA (DAPI, blue). Cilia are less abundant in Tmem67−/− tubules, but possess Arl13b. (g) Tmem67−/− primary MEFs generate cilia, as stained for AcTub (green), γ-tubulin (red), and DNA (DAPI, blue). (h) Tctn1 is epistatic to Tmem67. At E14.5, Tmem67−/− mice are indistinguishable from wild type, and Tctn1−/− Tmem67−/− double mutant mice are indistinguishable from Tctn1−/− mice. Scale bars 10μm.