Skip to main content
. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Hepatology. 2011 Jun 26;54(2):425–433. doi: 10.1002/hep.24399

Fig. 5. Apoptosis induction in Huh-7.5.1 cells transfected with JFH-1/wt, S2, and their chimeric constructs.

Fig. 5

(A) Three million cells were transfected with 3 µg in vitro transcribed full-genome RNA of JFH-1/wt, S2, S2-wt, and wt-S2. Apoptosis was induced by exposing cells to 20 ng/mL TNF-α plus 50 ng/mL Act D and detected by anti-cleaved PARP staining. The clone names and apoptosis induction indexes are indicated in the upper right box. (B) Apoptosis induction indexes of JFH-1/wt-, S2-, S2-wt-, and wt-S2- transfected cells. Means ± standard deviations of 3 independent experiments are shown. *p < 0.05 compared to JFH-1/wt.