Table 1.

Infectious virus production and release of JFH-1/wt and variants in Huh7–25 cells

Strain	Intracellular			Extracellular	Secretion Ratio (extra-/intra-)
	HCV RNA (copies/ µg RNA)	Infectivity Titer (ffu/well)	Specific Infectivity (ffu/copies)	Infectivity Titer (ffu/well)
JFH-1/wt	7.75 × 108 ± 1.04 × 108	4.21 × 102 ± 4.32 × 101	2.09 × 10−7 ± 7.06 × 10−8	1.94 × 103 ± 3.76 × 101	4.6 ± 1.3
JFH-1/S1	7.04 × 108 ± 8.49 × 107	4.72 × 102 ± 5.63 × 101	2.91 × 10−7 ± 6.00 × 10−8	3.02 × 103 ± 2.77 × 102	5.4 ± 2.0
JFH-1/S2	4.16 × 108** ± 7.47 × 106	5.19 × 103** ± 8.24 × 101	3.76 × 10−6** ± 7.01 × 10−7	3.23 × 104** ± 3.52 × 103	6.2 ± 3.0
JFH-1/C	3.15 × 108* ± 5.02 × 107	8.59 × 102* ± 4.81 × 101	8.19 × 10−7* ± 5.68 × 10−8	3.68 × 103 ± 3.02 × 103	4.3 ± 1.4
JFH-1/ S2-wt	7.07 × 108 ± 8.43 × 107	4.40 × 103* ± 9.5 × 101	2.73 × 10−6* ± 2.35 × 10−7	3.0 × 104* ± 1.1 × 103	6.7 ± 0.7
JFH-1/ wt-S2	4.21 × 108* ± 1.97 × 107	2.7 × 102 ± 2.9 × 101	2.02× 10−7 ± 4.0 × 10−8	1.7 × 103 ± 1.3 × 102	4.5 ± 0.4

^*p < 0.005 compared with JFH-1/wt

^**p < 0.005 compared with JFH-1/S1