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. Author manuscript; available in PMC: 2012 Aug 21.
Published in final edited form as: Lab Chip. 2011 Jul 14;11(16):2673–2679. doi: 10.1039/c1lc20151d

Figure 2.

Figure 2

Schematic procedure for on-chip FISH. (A) Cell loading. Microbial cells loaded into the cell reservoir are mobilized into the FISH chamber under an electric field of 20 V/cm. The direction of the electric field is switched every 30 seconds, alternating cell loading between the two membranes. A total 10 cycles are performed. (B) Probe loading. The probes in the probe reservoir are loaded into the FISH chamber following the same method as that of cell loading, except that probes are not loaded towards the washing membrane. Twenty loading cycles are conducted, during which probes are concentrated against the loading membrane. (C) Incubation. The loaded cells and probes are incubated together near the loading membrane. After every 290 s of incubation, the mixture is pushed against the loading membrane for 10 s. (D) Washing. The cells are moved to the washing membrane in this step. Excess probes are washed through the membrane under an electric field, or diffused out of the chamber via channel inlet/outlet. The total analysis time of the on-chip FISH is about 2.5 hours.