Fig. 4.

Phylogenetic tree of cell-envelop proteinases (CEP) of selected LAB. Deduced amino acid sequences of CEPs identified in other LAB were aligned using ClustalW [42], and the phylogenetic tree was calculated and visualized using Mega3.1 [40]. The evolutionary history was inferred using the neighbor-joining method [59]. The evolutionary distances were computed using the Poisson correction method [73] and are in the units of the number of amino acid substitutions per site. Protein names and respective organisms are indicated on the phylogenetic tree (color figure online)