Table 2.
Overview of human studies that demonstrate an association between IBS and compositional dysbiosis of the intestinal microbiota determined with culture-independent methods
| Study material | Population | Analytical methods | References |
|---|---|---|---|
| Faeces (3 time points) |
27 IBS patients 22 Healthy individuals |
qPCR | Malinen et al. (2005)* |
| Faeces (3 time points) |
26 IBS patients 25 Healthy individuals |
Conventional culturing DGGE Clone library sequencing (16S) |
Mättö et al. (2005)* |
| Biopsies: inflamed and non-inflamed tissue (ileum, ascending/sigmoid colon) |
20 CD patients 20 UC patients 20 Self-limiting colitis patients 20 IBS patients 20 Healthy individuals |
FISH | Swidsinski et al. (2005) |
| Faeces (2 time points) |
16 IBS patients 16 Healthy individuals |
DGGE TRAC |
Maukonen et al. (2006)* |
| Faeces |
24 IBS patients 23 Healthy individuals |
G+C based profiling Clone library sequencing (16S) qPCR |
Kassinen et al. (2007)* |
|
Duodenal biopsies Faeces |
41 IBS patients 26 Healthy individuals |
FISH qPCR |
Kerckhoffs et al. (2009)# |
| Faeces |
10 (+2) IBS (only IBS-D) 23 Healthy individuals |
G+C based profiling Clone library sequencing (16S) qPCR |
Krogius–Kurikka et al. (2009)* |
| Faeces (3 time points) |
20 IBS patients 15 Healthy individuals |
qPCR | Lyra et al. (2009)* |
|
Colonic biopsies Faeces |
10 IBS patients (only IBS-D) 10 Healthy individuals |
Conventional culturing qPCR |
Carroll et al. (2010) |
|
Colonic biopsies Faeces |
47 IBS patients 33 Healthy individuals |
DGGE | Codling et al. (2010) |
|
Duodenal biopsies Faeces |
37 IBS patients 20 Healthy individuals |
DGGE Clone library sequencing (16S) qPCR |
Kerckhoffs et al. (2010)# |
| Faeces | 44 IBS patients | qPCR | Malinen et al. (2010)* |
| Faeces |
26 IBS patients 26 Healthy individuals |
Conventional culturing qPCR HPLC |
Tana et al. (2010) |
All studies have applied Rome II or III criteria to recruit their subjects and categorise them in IBS subtypes. Studies that have used subjects from the same cohort are indicated by * and #
DGGE denaturing gradient gel electrophoresis, FISH fluorescence in situ hybridisation, HPLC high-performance liquid chromatography, qPCR quantitative polymerase chain reaction, TRAC transcript analysis with the aid of affinity capture