Figure 1.

Shear stress leads to increased membrane-associated Septin-2 in primary human bronchial epithelial (NHBE) cells. (A) NHBE cells were placed under shear stress, as described in Materials and Methods, for 2 hours and membrane preparations were compared with control cells under static condition. Immunoblotting shows that shear stress increases the membrane fraction of septin-2 in NHBE cells (left), whereas there is no change in septin-2 abundance in the whole-cell lysate (47). (B) Confirmation of shear-induced increases in membrane septin-2 expression is visualized using confocal immunofluorescence.